@article {573591, title = {Localization of antigenic determinants in whole Drosophila embryos.}, journal = {Dev Biol}, volume = {99}, number = {1}, year = {1983}, month = {1983 Sep}, pages = {261-4}, abstract = {Procedures are described for removal of the vitelline membrane from gently fixed Drosophila embryos en masse. The resulting embryos retain excellent structural integrity and are now suitable for a variety of immunocytochemical and biochemical characterizations.}, keywords = {Animals, Antibodies, Monoclonal, Drosophila melanogaster, Embryo, Nonmammalian, Epitopes, Fixatives, Fluorescent Antibody Technique}, issn = {0012-1606}, author = {Mitchison, T. J. and Sedat, J} }