@article {572451,
	title = {Dopamine receptor D3 regulates endocytic sorting by a Prazosin-sensitive interaction with the coatomer COPI.},
	journal = {Proc Natl Acad Sci U S A},
	volume = {109},
	number = {31},
	year = {2012},
	month = {2012 Jul 31},
	pages = {12485-90},
	abstract = {Macromolecules enter cells by endocytosis and are sorted to different cellular destinations in early/sorting endosomes. The mechanism and regulation of sorting are poorly understood, although transitions between vesicular and tubular endosomes are important. We found that the antihypertensive drug Prazosin inhibits endocytic sorting by an off-target perturbation of the G protein-coupled receptor dopamine receptor D(3) (DRD3). Prazosin is also a potent cytokinesis inhibitor, likely as a consequence of its effects on endosomes. Prazosin stabilizes a normally transient interaction between DRD3 and the coatomer COPI, a complex involved in membrane transport, and shifts endosomal morphology entirely to tubules, disrupting cargo sorting. RNAi depletion of DRD3 alone also inhibits endocytic sorting, indicating a noncanonical role for a G protein-coupled receptor. Prazosin is a powerful tool for rapid and reversible perturbation of endocytic dynamics.},
	keywords = {Adrenergic alpha-1 Receptor Antagonists, Biological Transport, Active, Coat Protein Complex I, Endocytosis, Endosomes, HeLa Cells, Humans, Prazosin, Protein Stability, Receptors, Dopamine D3},
	issn = {1091-6490},
	doi = {10.1073/pnas.1207821109},
	author = {Zhang, Xin and Wang, Wenchao and Bedigian, Anne V and Coughlin, Margaret L and Mitchison, Timothy J. and Eggert, Ulrike S}
}
