@article {1062471,
	title = {27 T ultra-high static magnetic field changes orientation and morphology of mitotic spindles in human cells},
	journal = {Elife},
	volume = {6},
	year = {2017},
	month = {2017 Feb 28},
	abstract = {Purified microtubules have been shown to align along the static magnetic field (SMF) in vitro because of their diamagnetic anisotropy. However, whether mitotic spindle in cells can be aligned by magnetic field has not been experimentally proved. In particular, the biological effects of SMF of above 20 T (Tesla) have never been reported. Here we found that in both CNE-2Z and RPE1 human cells spindle orients in 27 T SMF. The direction of spindle alignment depended on the extent to which chromosomes were aligned to form a planar metaphase plate. Our results show that the magnetic torque acts on both microtubules and chromosomes, and the preferred direction of spindle alignment relative to the field depends more on chromosome alignment than microtubules. In addition, spindle morphology was also perturbed by 27 T SMF. This is the first reported study that investigated the cellular responses to ultra-high magnetic field of above 20 T. Our study not only found that ultra-high magnetic field can change the orientation and morphology of mitotic spindles, but also provided a tool to probe the role of spindle orientation and perturbation in developmental and cancer biology.},
	issn = {2050-084X},
	doi = {10.7554/eLife.22911},
	author = {Zhang, Lei and Hou, Yubin and Li, Zhiyuan and Ji, Xinmiao and Wang, Ze and Wang, Huizhen and Xiaofei Tian and Yu, Fazhi and Yang, Zhenye and Pi, Li and Mitchison, Timothy J. and Lu, Qingyou and Zhang, Xin}
}
