Electron microscopy of microtubule cytoskeleton assembly in vitro.

Citation:

Coughlin M, Groen AC, Mitchison TJ. Electron microscopy of microtubule cytoskeleton assembly in vitro. Methods Mol Biol. 2014;1117 :259-71.

Date Published:

2014

Abstract:

Cell-free cytoplasm isolated from meiotic Xenopus egg extracts reconstitutes microtubule phenomena in vitro. These crude extracts assemble bipolar meiotic spindles and are readily fractionated for biochemical assays, providing a good tool to dissect molecular mechanism. We developed techniques for immunoelectron microscopy of microtubule structures assembled in perfusion chambers and in solution.